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Figure 6
Example curve-fitting of the observed transient rates of alkylenzyme formation and decay. (a, b) The substrate-dependence of the observed transient rates, kobs, in the hydrolysis of (S,S)-1a catalyzed by (a) R-C1 and (b) R-C1B1. In the case of R-C1, the obtained fluorescence traces were fitted to a second-order exponential (3)[link] to yield two observed rates (unfilled and half-filled circles, respectively), while the R-C1B1-catalyzed rates could be fitted to a first-order exponential (2)[link]. The substrate-dependencies of the respective kobs values were fitted to (4)[link] in the cases of a hyperbolic dependency [unfilled symbols in (a) and (b)] and to (5)[link] in the case of a decreasing kobs with an increasing concentration of substrate [half-filled symbols in (a)]. (c) The substrate-dependence of the observed transient rates, kobs, in the hydrolysis of (R,R)-1a catalyzed by R-C1 and R-C1B1 (inset). The observed concentration-dependency in the R-C1B1-catalyzed reaction was inconclusive and no fitting was performed. In the case of R-C1, the obtained kobs values were fitted to (5)[link]. See §[link]2 for details.

IUCrJ
Volume 5| Part 3| May 2018| Pages 269-282
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518003573