Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome

Woitowich, N.C.; Halavaty, A.S.; Waltz, P.; Kupitz, C.; Valera, J.; Tracy, G.; Gallagher, K.D.; Claesson, E.; Nakane, T.; Pandey, S.; Nelson, G.; Tanaka, R.; Nango, E.; Mizohata, E.; Owada, S.; Tono, K.; Joti, Y.; Nugent, A.C.; Patel, H.; Mapara, A.; Hopkins, J.; Duong, P.; Bizhga, D.; Kovaleva, S.E.; St. Peter, R.; Hernandez, C.N.; Ozarowski, W.B.; Roy-Chowdhuri, S.; Yang, J.-H.; Edlund, P.; Takala, H.; Ihalainen, J.; Brayshaw, J.; Norwood, T.; Poudyal, I.; Fromme, P.; Spence, J.C.H.; Moffat, K.; Westenhoff, S.; Schmidt, M.; Stojkovic, E.A.

doi:10.1107/S2052252518010631

Figure 4
Absorption spectra of various SaBphP1 PCM constructs where the Pr to Pfr transition is initiated with 700 nm light. Black solid line, 20 min of 740 nm; dashed line, 20 min of 700 nm illumination; except (d) where illumination is for an hour. The location of the residues can best be identified in, and their function inferred from Figs. 6

and 9

. (a) wild-type, (b) T289H mutant, (c) D208T, T289H double mutant, (d) Y262F, T289H double mutant. Black solid line, dark adapted and 1 h of 700 nm illumination is identical; dashed line, 1 h of 750 nm illumination, (e) R472A mutant, (f) S747A mutant.

IUCrJ

Volume 5| Part 5| September 2018| Pages 619-634

ISSN: 2052-2525

https://doi.org/10.1107/S2052252518010631

BIOLOGY | MEDICINE

Open

access

Follow IUCrJ

Search IUCr Journals		doi		Advanced search
Author		volume	page