Single-particle imaging without symmetry constraints at an X-ray free-electron laser

Rose, M.; Bobkov, S.; Ayyer, K.; Kurta, R.P.; Dzhigaev, D.; Kim, Y.Y.; Morgan, A.J.; Yoon, C.H.; Westphal, D.; Bielecki, J.; Sellberg, J.A.; Williams, G.; Maia, F.R.N.C.; Yefanov, O.M.; Ilyin, V.; Mancuso, A.P.; Chapman, H.N.; Hogue, B.G.; Aquila, A.; Barty, A.; Vartanyants, I.A.

doi:10.1107/S205225251801120X

Figure 4
(a)–(c) FV densities on PCA planes for different intensity thresholds (number of hits in Table 1

). (d)–(f) Projection view of the PCA densities with manually classified single-hit patterns shown as red dots. Blue and yellow dots correspond to the same selections as in Fig. 3

. The black contour level corresponds to 3.3% of the maximum value of the PCA density (selected hits in Table 1

). For low-intensity thresholds, the black contour contains a region that is not clearly represented by the manual single hit selection (a), (d) and (b), (e). The manual hit selection is most precisely matched by data Set_10k^PCA within the contour line for an intensity threshold at 2 × 10⁵ ph in (c) and (f).

IUCrJ

Volume 5| Part 6| November 2018| Pages 727-736

ISSN: 2052-2525

https://doi.org/10.1107/S205225251801120X

PHYSICS | FELS

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