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Figure 4
Molecular interactions of vPIP mutants with PARP-1 or RTA. (a) Interaction between vPIP mutants and PARP-1. MYC-tagged vPIP mutants were co-transfected with FLAG-tagged PARP-1 into HEK293T cells. The cells were harvested 48 h post-transfection and analyzed by co-IP assays with the anti-FLAG antibody. The results were examined by Western blotting. The band intensity of MYC in IP blots was measured using the ImageJ software. (b) Inhibition of interactions between RTA and PARP-1 by vPIP mutants. MYC-tagged vPIP mutants were co-transfected with FLAG-tagged RTA into HEK293T cells. The cells were harvested 48 h post-transfection and subjected to co-IP assays with the anti-FLAG antibody. The results were examined by Western blotting. The band intensity of PARP-1 and MYC in IP blots was measured in ImageJ. (c) A summary of subcellular localization, protein–protein interactions and functional activities of WT vPIP and mutants.

IUCrJ
Volume 5| Part 6| November 2018| Pages 866-879
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518013854