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Figure 2
SANS data for GluA2 in the resting state (apo). (a) Experimental data (grey points), the resulting fits and residual plots, fitted with the X-ray crystal structure of GluA2 in the resting state (PDB entry 4u2p; cyan; Dürr et al., 2014BB4) and the EM structure of GluA2 with quisqualate (class 3; EMD-2688; magenta; Meyerson et al., 2014BB30). A cartoon representation of the crystal structure of GluA2 is shown in cyan and an atomic model fitted into the class 3 EM structure is shown in magenta. (b) Pair distance distribution function [p(r)] for the experimental GluA2 data in solution (grey points) and theoretical p(r) functions for the GluA2 crystal structure in the resting state (cyan) and for the GluA2 class 3 EM structure (magenta). (c) Experimental SANS data and the fit of a linear combination of the crystal structure of GluA2 in the resting state and fractal oligomers (black), and a linear combination of the crystal structure and the class 3 EM structure (green). (d) Pair distance distribution functions for the fits. (e) Ab initio model generated with DAMMIF. The size of the beads was weighted by occupancy. (f) The GluA2 crystal structure in the resting state (PDB entry 4u2p; cyan) was manually overlaid with the DAMMIF envelope (grey).

IUCrJ
Volume 5| Part 6| November 2018| Pages 780-793
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518012186