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Figure 4
Comparison of the parent, Tako and Ika propeller structures. (a) The overall β-propeller domain is depicted as a cartoon coloured from blue to red from the N-terminus to the C-terminus. (b) Superposition of the individual blades of the different proteins indicates that the parent protein does not exhibit a conserved backbone geometry, while the eight blades of Tako8 can be perfectly aligned. In the case of Ika8 only the alternating a and b repeats can be perfectly aligned. The differences in symmetry are also clear from the electrostatic potential as calculated by APBS (Touw et al., 2015BB50; Kabsch & Sander, 1983BB21; Jurrus et al., 2018BB18) (c). In the case of Tako8 the interior of the toroid is clearly highly negatively charged, while for Ika8 the charges are more evenly distributed.

ISSN: 2052-2525