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Figure 4
Confidence maps from subtomogram averages. (a) Nuclear pore structure at 90 Å (EMD-8055) from eight pore particles: cryo-EM map at 2.0σ threshold (left, gray) and confidence map at 1% FDR threshold (right, blue). Note that the confidence map minimizes the appearance of noise. (b) ER-associated ribosome structure at 35 Å resolution (EMD-8056) in two side views at a 0.8σ threshold (left) and 1% FDR confidence map (right). Note that in confidence maps weaker densities assigned to the peripheral protein complexes TRAP and OST (arrows) can easily be visualized in the absence of noise.

IUCrJ
Volume 6| Part 1| January 2019| Pages 18-33
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518014434