Figure 6
Superposition of the coordinates of the two HEWL structures obtained by 3D ED (C atoms in violet) and X-ray diffraction on the microfocus beamline (C atoms in orange). The insets on the right show the electron-density map in the region of the Thr47-loop (top panel, map calculated by exclusion of the missing Fobs; bottom panel, map calculated by replacing the missing Fobs with Fcalc); the inset on the left shows minor structural changes of the protein localized at the Thr47-loop, where the protein seems to be more affected by the crystal-growth process. |