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Figure 6
NMR characterization of PAF–sclxn­ complexation. (a) Region from overlaid 1H-15N HSQC spectra of pure PAF (black contours) and in the presence of 0.1–0.6 mM ligand (coloured scale). Biphasic shifts occurred for resonances Lys30, Lys34 and Cys36 in the presence of sclx6. Resonances Lys11, Cys28, Lys30, Lys34 and Cys36 were broadened at 0.3 mM sclx8, while resonances Thr8, Lys11, Asp32 and Thr37 were broadened beyond detection at 0.6 mM sclx8. (b) Plots of chemical-shift perturbations measured for PAF backbone amides in the presence of 0.6 mM sclx4, sclx6 or sclx8. Blanks correspond to Pro30 and undetectable resonances (due to broadening).

IUCrJ
Volume 6| Part 2| March 2019| Pages 238-247
ISSN: 2052-2525
https://doi.org/10.1107/S2052252519000411