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Figure 1
Ternary complex structure of T4dCH with dCMP and THF. (a) Ribbon diagram showing the ternary complex structure of dimeric T4dCH viewed along the crystallographic twofold symmetry axis. N- and C-terminal ends are colored red and labeled as Nt and Ct, respectively, Secondary structural elements are colored green (α-helix and 310-helix), purple (β-strand) and cyan (loops). The bound dCMP (yellow) and THF (orange) are drawn using the ball and stick model. The bound iodide is also shown as a gray ball. (b) Electron-density maps showing the bound dCMP (yellow) and THF (orange) in the active site of T4dCH. The 2Fo − Fc map (blue) is contoured at 1.0σ. (c) Ball-and-stick model of dCMP (yellow) and THF (orange) in the deep binding pocket, which is represented as an electrostatic surface. The positively and negatively charged areas are blue and red, respectively. Each subpart of THF is labeled with black dotted circles. (d) B factor diagram of the apo (green), binary (cyan) and ternary complex (blue) forms. The thickness of the tube reflects the value of the B factor, i.e. the higher the B factor, the thicker the tube.

IUCrJ
Volume 6| Part 2| March 2019| Pages 206-217
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518018274