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Figure 4
Reaction mechanism of T4dCH. (a) Schematic diagram showing the proposed mechanism of cofactor activation. For methyl-group transfer, the mTHF is converted to protonated mTHF+ by a water (Wat10) that had been activated by Glu60. The electron flows are indicated by arrows. (b) Schematic diagram showing the proposed mechanism of hydroxy­methylation by T4dCH. The intermediates 1, 2, 3 and 4 are shown. The hydrogen-bonding interactions are represented as dotted lines.

IUCrJ
Volume 6| Part 2| March 2019| Pages 206-217
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518018274