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Figure 4
Deep-UV-laser machine setup and laser shaping of lysozyme crystal into spheres of different diameters. (a) Schematic diagram of the deep-UV-laser machine along with focusing optics, goniometer and cryojet. (b) Top-view of the real-life setup of the deep-UV-laser system at SPring-8, Japan. (c) Original large lysozyme crystal of 800 × 500 × 400 µm (before laser cutting) and the white contour was a template made for a spherical shape with precise diameters of each of the spheres, including the error margin for the laser-cutting process. (d) The same crystal after laser cutting with a deep-UV laser of wavelength 193 nm. The size of each sphere is written in red and the diameters are marked with corresponding red lines. There are four spheres – two of 50 µm, one of 100 µm and one of 200 µm diameter. The part of the crystal on the extreme right is the `unshaped' region, along with the base of the original loop. A supplementary movie of the laser-cutting process is also available in the Supporting information.

IUCrJ
Volume 6| Part 3| May 2019| Pages 373-386
ISSN: 2052-2525
https://doi.org/10.1107/S2052252519002756