Cryo-EM density of TpH. (a) Representative cryo-EM micrograph of vitrified TpH (scale bar: 50 nm). (b) Representative class averages (scale bar: 20 nm). (c) Cryo-EM structure of TpH refined with C5 symmetry imposed. (d) The ab initio model calculated using RVIPER with no symmetry imposed. (e) Final cryo-EM structure of TpH refined with no symmetry imposed. The wall region, FU-gs and FU-d*s are shown in gray, cyan and yellow, respectively. Cut-open views are also shown.