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Figure 1
Mean intensity, completeness and multiplicity as a function of exposure. (a) For each camera, the integrated intensities on the subset of frames in the (−30°, +30°) tilt range were averaged; this reduces systematic effects on the intensities arising from longer paths through the sample at higher tilt. The reflections in the resolution range common to all data sets (20.0–2.70 Å) were then fitted to a function of the form Acryst exp(−Bcam × x), where Acryst was refined for each crystal and Bcam was refined for each camera. The dotted vertical lines indicate the exposure at which 95% completeness was obtained. (b) The exposure-dependency of the completeness was determined by merging only frames with an average exposure less than the given value. The dotted horizontal line marks 95% completeness. (c) In all cases, the multiplicity increases approximately linearly with dose, which implies that completeness is indicative of the amount of information recovered at the given dose.

Volume 6| Part 5| September 2019| Pages 921-926
ISSN: 2052-2525