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Figure 6
The relationship between crystal volume, obtained resolution and molecular weight. The crystal volume is plotted against the highest resolution cutoff from autoprocessing and is coloured by the molecular weight of the entity crystallized; note the logarithmic scales for volume and molecular weight. Lines describe the minimum crystal volume required for a certain resolution given the parameters shown assuming a Nave–Hill effect (photoelectron escape) of 1, as all crystals are larger than 1 µm3, and 100 photons per hkl (equation 16 from Holton & Frankel, 2010BB29). The lines indicate a specific molecular weight and B factor and should be taken as a guide to the rough range described. The wavelength on MASSIF-1 is fixed and the beam diameter is altered to match the crystal. The histogram shows the distribution of resolutions obtained in the scatter plot. N = 15 241.

Volume 6| Part 5| September 2019| Pages 822-831
ISSN: 2052-2525