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Figure 2
Absorption spectra of Ngb in crystallo at 15 and 30 K. (a) In crystallo UV–visible spectra of ferric (Fe3+) Ngb (black line) and hexacoordinate ferrous (Fe2+) Ngb (red line) were recorded at 15 K. Hexacoordinate ferrous (Fe2+) Ngb crystals were obtained by soaking in mother liquor containing sodium dithionite, a reducing agent. (b) It is possible to trap the photolytic intermediate Ngb*CO (cyan line) at 15 K under continuous illumination using the probing lamp of the microspectrophotometer. The 15–30 K temperature jump is associated with the geminate recombination of CO to the heme Fe atom (green line).

Volume 6| Part 5| September 2019| Pages 832-842
ISSN: 2052-2525