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Figure 3
Interaction between GST domains via Interfaces 1 and 2. (a) Two DA2E subcomplexes within an asymmetric unit show an overall twofold rotational symmetry. One of the subcomplexes is shown in paler colors to distinguish the two. (b) Close-up view of the heterodimeric AIMP2GST–EPRSGST complexes at the interface of the two DA2E subcomplexes. AIMP2GST and EPRSGST interact via Interface 1, while two EPRSGST molecules from adjacent subcomplexes interact via Interface 2. (c) Close-up view of the model structure of heterotetrameric AIMP2GST–EPRSGST–AIMP3–MRSGST built upon the DA2E subcomplex structure, in which AIMP3 and MRSGST are colored violet and red, respectively (PDB entry 4bvx). The assembly of the heterotetrameric GST domains is identical to that of AIMP2GST–EPRSGST–EPRSGST–AIMP2GST observed in our structure (b). (d) Sequence alignment of residues in Interface 2 of EPRSGST and AIMP3 using Clustal Omega (Sievers et al., 2011BB38) displayed with ESPript 3 (Robert & Gouet, 2014BB33). The secondary-structure elements were defined based on the structure of EPRSGST. Conserved residues and similar residues are highlighted in red and yellow, respectively.

Volume 6| Part 5| September 2019| Pages 958-967
ISSN: 2052-2525