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Figure 4
Model building of the MSC subcomplex based on the DA2E structure. (a) Schematic of the AIMP2 sequence showing multiple protein-binding sites as a scaffold protein. Notable structural motifs are indicated within the schematic. The names of the proteins are listed below the region of AIMP2 to which they bind. The site of phosphorylation (Ser156) is marked with a red circle. (b) Building the DA2EA3M structure model onto the DA2E structure. The structures of EPRSGST–AIMP3 (PDB entry 5bmu) and AIMP3–MRSGST (PDB entry 4bvx) complexes were sequentially added to the DA2E structure by superposition. The yeast tRNAAsp structure was laid onto the model by superimposing the structure of yeast DRS in complex with yeast tRNAAsp (PDB entry 1asy; Ruff et al., 1991BB35). (c) Structure of the extended MSC model including DA2EA3M and RQA1 (PDB entry 4r3z) subcomplexes and homodimeric KRS structures (PDB entry 4dpg). Each of the components were assigned with respect to where they would bind to AIMP2, which is represented as a cartoon model. AIMP2 segments are shown in surface representation. A symmetry-related duplicate of the extended model is shown as a ribbon representation. Flexible regions of AIMP2 and EPRS are represented by orange and blue dashed lines, respectively. The coloring scheme is as follows: DRS, green; yeast tRNAAsp, cyan; AIMP2, orange; EPRS, blue; AIMP3, violet; MRS, red; RRS, gray; QRS, purple; AIMP1, yellow; KRS, brown.

Volume 6| Part 5| September 2019| Pages 958-967
ISSN: 2052-2525