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Figure 4
Pro13 is essential for the quaternary stability of CFA/I pili. Mutation of Pro13 to phenyl­alanine in CfaB changed the force required to unwind the quaternary structure of a pilus. (a) Force response of a single wild type CFA/I pilus (black) and of a single mutated CFA/I pilus (blue). (b) Histogram of the wild type (gray) and mutant (blue) unwinding forces. (c) Extension (black) and rewinding (light blue) of a single mutated CFA/I pilus.

IUCrJ
Volume 6| Part 5| September 2019| Pages 815-821
ISSN: 2052-2525
https://doi.org/10.1107/S2052252519007966