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Figure 2
Pull-down assay and size-exclusion chromatography for pairs of archaeal ScpA and ScpB homologues. The name of the organism from which the two proteins were derived is shown at the top of each panel. Denaturing gels and chromatograms are shown on the left and right, respectively. (His)10-MBP-tagged ScpA proteins were mixed with the indicated ScpB homologues (TON_1955, PYCH_12850, MCON_2432 and Mzhil_1996) in a 1:1 molar ratio (1 µM each). `I' and `R' stand for the input proteins loaded onto the Ni–NTA resin and the resin-bound proteins, respectively. Molecular weights are labelled in kDa. For chromatography, individual proteins or their mixture (100 µg each) were loaded onto an analytical gel-filtration column. The two black triangles at the top of the chromatograms indicate the elution positions of the molecular-weight markers: 158 kDa (left) and 43 kDa (right).

IUCrJ
Volume 7| Part 2| March 2020| Pages 193-206
ISSN: 2052-2525
https://doi.org/10.1107/S2052252519016634