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Figure 4
Multiple sequence alignment of ScpAs from archaea and bacteria. ScpA sequences derived from nine archaeal species and four bacterial species were aligned using Clustal Omega (Sievers et al., 2011BB30). Secondary-structural elements of ToScpAN and GsScpAΔC are indicated at the top and the bottom of the alignment, respectively. The black box indicates the region of ScpAN that shows a clear difference between archaeal and bacterial ScpA proteins. T.onnurin, Thermococcus onnurineus (gi:212009177); M.arvoryz, Methanocella arvoryzae (gi:500971514); M.barkeri, Methanosarcina barkeri (gi:805410469); M.zhilina, Methanosalsum zhilinae (gi:335931289); M.liminat, Methanofollis liminatans (gi:395441815); M.formici, Methanotorris formicicus (gi:373561535); G.acetivo, Geoglobus acetivorans (gi:728876205); T.acidoph, Thermoplasma acidophilum (gi:851298378); M.termitu, Candidatus Methanoplasma termitum (gi 851220379); M.washbur, Candidatus Methanodesulfokores washburnensis (gi:1538762872); M.acidiph, Candidatus Mancarchaeum acidiphilum (gi:1214173397); N.viennen, Nitrososphaera viennensis (gi:647811207); N.sedimin, Candidatus Nitrosopumilus sediminis (gi:407047581); R.qingshe, Rhodococcus qingshengii (gi:1595910292); P.aerugin, Pseudomonas aeruginosa (gi:1440714951); T.roseus, Terriglobus roseus (gi:1124365071); G.stearot, Geobacillus stearothermophilus (gi:1017231538). For expanded and grouped sequence alignments, see Supplementary Figs. S1 and S2.

IUCrJ
Volume 7| Part 2| March 2020| Pages 193-206
ISSN: 2052-2525