Evidence for binary Smc complexes lacking kite subunits in archaea

Jeon, J.-H.; Lee, H.-S.; Shin, H.-C.; Kwak, M.-J.; Kim, Y.-G.; Gruber, S.; Oh, B.-H.

doi:10.1107/S2052252519016634

Figure 5
Protein-binding assay for archaeal ScpAs with a longer middle region. The indicated ScpA and ScpB homologue were mixed together in a 1:1 molar ratio (1 µM each), loaded onto the resin and analyzed by denaturing gel electrophoresis (left) or on an analytical size-exclusion column (right). The name of the source organism is shown at the top. Molecular weights are labelled in kDa for the gels. The two black arrows indicate the elution positions of the molecular-weight markers. GACE_1479 (174 amino acids) appears as a monomeric protein and Metli_0606 (161 amino acids) as a smaller dimeric protein in comparison with the ScpB homologues in Fig. 2