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Figure 2
Comparison of FAS preparations. (a) The published map (D'Imprima et al., 2019BB7) lacks the PPT domain and parts of the β-domes are poorly resolved (red circles). (b) Data collected using protein prepared by the optimized protocol described here. The 2D class averages show structured PPT domains (blue arrows) and resolved secondary-structure features at the β-domes. (c) CryoEM map from 15 000 particles at 3.1 Å resolution.

Volume 7| Part 2| March 2020| Pages 220-227
ISSN: 2052-2525