Figure 1
Structure of Zn– and Cu–CAII: active site and water network. (a) Zn–CAII. The zinc metal is stabilized by three histidines (His94, His96 and His119). His64 is depicted in its dual `in' and `out' conformations. The N terminus (residues 1–4) is disordered. The substrate CO2 is shown bound adjacent to the zinc, stabilized by the hydrophobic pocket (Domsic et al., 2008). (b) Cu–CAII. The copper metal (T-2 site) is stabilized by the same three histidines as the zinc. Also, His64 was observed in dual conformations. The NO2− bound with an oxygen and a nitrogen interacting with the copper. The water network resembles that observed in Zn–CAII, with the exception of the extended water network (W4 and W5), creating a hydrogen-bonding network spanning from the N terminus to the active site. The N terminus is ordered, forming a pseudo-porphyrin ring around a second copper (T-2 site). The catalytic metals are depicted as spheres: zinc (magenta) and copper (brown). The hydrophobic residues (Ile91, Val121, Phe131, Val135, Leu141, Val143, Leu198, Pro202, Leu204, Val207 and Trp209) are highlighted as orange and the hydrophilic residues (Asn62, His64, Asn67, Gln92, Thr199 and Thr200) as violet. The active-site solvent network (W1, W2, W3a and W3b) is depicted as red spheres and the extended water network (W4 and W5) is shown in the Cu–CAII substituted structure. |