MicroED data collection. CypA crystals were deposited on a copper grid with an amorphous carbon support material and frozen in vitreous ice (left). A single crystal (highlighted in a red box in image 1) is shown at various stages (1–6) of the FIB-milling process. The edge of the final crystalline lamella is denoted with a red arrow in image 6. Also shown is the intensity-weighted reciprocal lattice (right) representing the MicroED data that were collected from the single crystal shown in the left panel.