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Figure 6
Visualization of the 2mFoFc map and the refined model measured from an FIB-milled crystal using MicroED. The conformation of residues coupled to the catalytic site resembles structures previously solved under cryogenic conditions using X-ray crystallography (PDB entry 3k0m). For some regions of the structure, the cryogenic X-ray and MicroED structures are indistinguishable. A previously published multi-conformer model produced from data acquired at room temperature is provided for comparison (PDB entry 3k0n). Following refinement, ensembles were generated using phenix.ensemble_refine. In the right panel, a histogram of the χ1 angles for residue 113 is plotted for the ensemble. All members of the ensemble adopted the same rotameric position as previous cryogenic structures.

Volume 7| Part 2| March 2020| Pages 306-323
ISSN: 2052-2525