Overview of the scanning NanoEDT experiment. (a) Schematic of the experimental geometry for collecting nanobeam electron-diffraction data with key components highlighted. (b) ADF image of a crystal of segment 24AVAAGA29 from the OsPYL/RCAR5 protein interrogated by an electron beam. The scale bar represents 400 nm. (c) Composite image of all the diffraction patterns collected simultaneously with the ADF image in (b). The red outline indicates the region of the image used to compute diffraction patterns. (d) Tomographic reconstruction of the crystal in (b). (e) Examples of diffraction images taken at discrete orientations during electron-diffraction tomography. (f) Atomic structure of the OsPYL/RCAR5 peptide 24AVAAGA29 solved by NanoEDT.