Fragment-based phasing of NanoEDT data. (a) The amyloid peptide fragment library used as input for ARCIMBOLDO. The final fragment placed and the structure it is derived from (Sawaya et al., 2016) are highlighted by the blue and black boxes, respectively. (b) LLG versus initial CC for all fragments used by ARCIMBOLDO to find the initial phasing solution. The colour bar represents the mean phase error of a given fragment compared with the final solution. (c) The initial fragment placed by ARCIMBOLDO (blue) overlaid on the final solution (purple). (d) The final refined structure of the OsPYL/RCAR5 peptide 24AVAAGA29. H atoms are shown in white and highlighted by black arrows. The blue mesh represents the 2Fo − Fc map (contoured at 1σ) and the green/red mesh represents the Fo − Fc map (contoured at ±3σ).