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Figure 3
The effect of BDE-100 on the verapamil-stimulated ATPase activity of the six Pgp mutations. The effect of BDE-100 in the absence of verapamil on each mutant was determined at 50, 75 and 100 µM. The EC50 of verapamil-stimulated ATPase activity of each mutant was pre-determined (Supplementary Fig. S4) and is shown here. The level of BDE-100 inhibition of the verapamil-stimulated ATPase EC50 for each mutant is also shown. P values were were determined from a two-tailed unequal variance test: ****, p ≤ 0.0001; ***, p ≤0.0001–0.001; *, p ≤0.05–0.01. The scale of each graph is represented to enhance the differences of verapamil concentration on ATPase for the given mutant, particularly since one mutant, F728A, exhibited a considerably lower Vmax compared with the other mutants.

Volume 7| Part 4| July 2020| Pages 663-672
ISSN: 2052-2525