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Figure 4
Dose–response curve of verapamil-stimulated ATPase activity. Curves for each different mutant are colored according to the legend of the figure. The F728A mutant exhibited the highest EC50 of 60 µM, followed by C952A (32 µM), F979A (30 µM), F724A (28 µM), Y306A (25 µM) and Y303A (7.3 µM). All measurements were determined with seven independent measurements (n = 7).

IUCrJ
Volume 7| Part 4| July 2020| Pages 663-672
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520005709