Figure 1
Crystal structure of N-Wag31. (a) Two nearly orthogonal views of the N-Wag31 (residues 2–60) dimer as blue and pale brown ribbons are shown. The calculated local coiled-coil axes are shown as red jointed lines. (b) A CD profile (molar-residue ellipticity versus wavelength) of N-Wag31. (c) Superimposed structures of N-Wag31 (blue) and N-bsDivIVA (coral) are shown. (d) The intertwined loop region in N-Wag31 (blue) containing the P16P17I18 sequence (balls and sticks, with carbon atoms in light blue, oxygen in red and nitrogen in blue) and the equivalent region in N-bsDivIVA (coral) containing the stacked Phe17 residues (balls and sticks, with carbon atoms in coral, oxygen in red and nitrogen in blue), are shown. (e) Multiple sequence alignment of the N-terminal region of tbWag31 (numbering for tbWag31 in red) and homologs, with the degree of conservation, is shown (prepared in Jalview; Waterhouse et al., 2009). |