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Figure 6
Snapshots of the molecular movie resulting from the TR-SOX experiment. (a) Snapshots at time points 0, 1, 3, 6, 10, 25 and 66 showing 2Fobs − Fcalc electron-density maps contoured at a 1.2σ level superimposed on the structural models of the dark and light states highlighting covalent bond formation between Cys426 and the FMN, disordering of the Gln489 side chain, and displacement of the Phe470 side chain. (b) A different view showing 2Fobs − Fcalc electron-density maps contoured at a 0.7σ level illustrating rotation of the isoalloxazine ring of the FMN, translation of Ile403 and progressive disappearance of the B conformation of Cys426.

IUCrJ
Volume 7| Part 4| July 2020| Pages 728-736
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520007411