Refined 3D maps for the six EMPIAR data sets. For each data set, refined maps were color coded based on local-resolution estimation. The size of each reconstruction roughly reflects its actual size relative to a ribosome. (a) β-Galactosidase (520 kDa), (b) 20S proteasome (700 kDa). (c) aldolase (150 kDa), (d) TRPV1 (280 kDa), (e) 80S ribosome (1263 kDa), (f) streptavidin (53 kDa). The keys indicate the resolutions of the colored maps: dark blue for higher resolution, and dark red for lower resolution.