Figure 4
Replicate VR titration curves of HisBP against four ligands: His (black), Arg (blue), Lys (violet) and Orn (red). Data collected at various total doses: (a) 1.6 × 1014 photons at Diamond, N = 192; (b) 2.6 × 1013 photons at DESY, N = 172; (c) 2.6 × 1013 photons at Australian Synchrotron, N = 144; and (d) 3 × 1012 photons at ESRF, N = 144. The HisBP concentrations used within each experiment are labelled on respective plots. Note that the maximum q-range measured in (d) was 2.8 nm−1, which affected the VR. |