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Figure 3
Improved correction of beam-induced motion with EER files. (a) Example of individual particle trajectories measured from 30 exposure fractions and interpolated to the physical frame rate of the camera. The yellow line represents the applied motion without the B-spline interpolation enabled by the EER method, while the blue line represents the interpolated trajectory enabled by EER. (b) Fourier shell correlation curve for 3D reconstructions without (black curve; 2.10 Å resolution at FSC = 0.143) and with (red curve; 2.07 Å resolution at FSC = 0.143) interpolated motion applied to the individual camera frames. (c) Comparison of resolution for 3D maps (FSC = 0.143) calculated from different exposure fractions, each corresponding to 0.7 e− Å−2, without (black curve) and with (red curve) interpolated motion applied to the camera frames.

IUCrJ
Volume 7| Part 5| September 2020| Pages 860-869
ISSN: 2052-2525
https://doi.org/10.1107/S205225252000929X