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Figure 3
Association of bR and OG in LCP. (a) SANS of a concentration series of bR/cmOG in LCP, compared with blank LCP. (b) Blank LCP curve subtracted to obtain the scattering from bR alone, which was compared with bR solution scattering. (c) Subtracted curves normalized by bR concentration and averaged. Error bars are the standard deviation of the distribution of individual normalized curves prior to averaging. This procedure was performed for bR/cmOG (concentrations of 0.66, 1.7 and 4.2 mg ml−1) and bR/hOG (1.3, 1.4, 2.1, 2.5, 3.2 and 5.2 mg ml−1). Contrast-matching of OG has no observable effect on the normalized scattering, indicating that OG is not associated with bR in any significant amount in the LCP. (d) Buffer-subtracted and normalized scattering of bR/cmOG (0.8, 1.7, 5.2, 6.8 and 10.4 mg ml−1) and bR/hOG (0.9, 1.1 and 1.47 mg ml−1) in solution. The intensity scale is the same as for (c). Unlike the LCP case, in solution, contrast-matching of OG causes a pronounced change in scattering intensity, indicating association of the detergent with bR.

IUCrJ
Volume 8| Part 1| January 2021| Pages 22-32
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520013974