Figure 1
Purification of an antifungal protein from S. cinnamonensis strain KPP02129. (a) Antifungal activity of semi-purified fractions. (b) Silver-stained SDS–PAGE during anion-exchange chromatography. The arrow indicates the purified protein. Lane M, marker; lane Ft, flowthrough fraction; lane W, washing fraction; lane E1, eluted fraction 1; lane E2, eluted fraction 2. (c) Streptavidin C1 was purified via gel-filtration chromatography. Arrowheads indicate the peak and bands corresponding to streptavidin C1. (d) Antifungal activities of the two proteins treated with or without D-biotin against F. oxysporum f. sp. cucumerinum. |