view article
Figure 2
Molecular mass and thermal stability of biotin-binding proteins. (a) Streptavidin C1 (blue line), streptavidin C2 (red line), ΔC1 (silver line) and ΔC2 (yellow line) analyzed by SEC-MALS. All samples were measured at a concentration of 1–2 mg ml−1. The horizontal line represents the measured molar mass. Experimental (MALS) molar-mass values are indicated as arrows. (b) Thermal unfolding profile analysis of streptavidin C1 (violet), streptavidin C2 (red), streptavidin (green) and avidin (brown) with or without biotin from differential scanning fluorimetry.

IUCrJ
Volume 8| Part 2| March 2021| Pages 168-177
ISSN: 2052-2525
https://doi.org/10.1107/S2052252520015675