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Figure 3
The changing background in AEX-SAXS can be complex. (a) Comparison of χ2 values from the REGALS deconvolution of the AEX-SAXS dataset in Fig. 2[link] with two background components (top panel, blue) versus one (bottom panel, orange). The former is relatively uniform around the expected value of 1, whereas the latter shows unevenness throughout the elution (black curves are the smoothed χ2 values shown as trend lines), indicating that dataset is better described with two background components. (b) Because the background scattering includes significant low-q features, failing to take proper account of the changes in the background can lead to artifacts in the extracted protein scattering profiles. Here, the use of only one background component in the analysis leads to a downturn in the low-q region of the scattering from component 1, which will lead to an underestimation of the protein size.

IUCrJ
Volume 8| Part 2| March 2021| Pages 225-237
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521000555