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Figure 6
Separation of changes at different length scales in a time-resolved T-jump dataset in REGALS. (a) Difference scattering from a previously reported time-resolved T-jump experiment on CypA (Thompson et al., 2019BB39) as a function of time delay (violet to red). (Inset) The mean intensity ΔI over q = 0.03–0.05 Å−1 increases before decreasing. (b) Regularized ΔP(r) functions from REGALS deconvolution. Three cut-offs were chosen to separate changes at different length scales: the equilibrium CypA structure (49 Å, orange), the thermally excited intermediate (59 Å, blue) and the large length-scale change (150 Å, green). (c) Concentration profiles from REGALS deconvolution (continuous curves) and unregularized concentrations (circles), showing different kinetics for conformational changes at different length scales. (d) Reconstructed ΔP(r, t) from deconvolved components, displaying two distinct processes occurring at small and large length scales.

IUCrJ
Volume 8| Part 2| March 2021| Pages 225-237
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521000555