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Figure 5
Averaged per-frame motion of all particles used in the last refinement round of (a) alcohol oxidase (AOX) and apoferritin (ApoF) (b, c). The particle drift is calculated from the particle trajectories determined by Bayesian polishing in RELION-3 (Suppplementary Fig. S3). A systematic reduction of particle drift is evident for both apoferritin and alcohol oxidase in cubic ice (red) compared with vitreous water (blue) in the most important early frames.

Volume 8| Part 2| March 2021| Pages 186-194
ISSN: 2052-2525