Figure 1
The overall structure and oligomeric state of MazEF (a) A cartoon representation of the MazEF heterohexamer. Chains A and D of the MazE antitoxin are shown in orange. Chains B, C, E and F of the MazF toxin are shown in light blue and green. (b) Superposed SEC chromatograms of full-length and truncated MazEF. (c) The standard curve of a HiLoad 16/600 Superdex 200 prep-grade column is shown as a red dotted line. The elution volumes of truncated and untruncated MazEF projected on this line allow the determination of the corresponding molecular masses. |