Figure 2
Hexameric structure of abMurG. (a) Crystallographic packing symmetry analysis. Three abMurG molecules in the asymmetric unit are indicated by color cartoons, whereas other symmetric molecules are indicated by gray ribbon structures. (b, c) Tentative hexameric structure of abMurG generated by the symmetry analysis: side (b) and top (c) views. (d, e) Multi-angle light scattering (MALS) profiles derived from the first (d) and second (e) size-exclusion chromatography peaks. The red line indicates the experimental molecular mass analyzed by MALS. (f) Schematic planar diagram showing the hexamer assembly strategy of two tentative dimers. |