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Figure 1
(a) Sodium do­decyl sulfate-polyacryl­amide gel electrophoresis analysis of the purification of CYP105D18 [1, crude extract; 2, insoluble fraction; 3, marker (kDa); 4, 10 mM imidazole fraction; 5, 100 mM first fraction; 6, 100 mM second fraction and 7, 500 mM imidazole fraction]. The 100 mM imidazole second fraction (sample 6) was concentrated for in vitro reaction. The molecular weight of CYP105D18 is 44.3 kDa, and the protein was overexpressed using the pET28 vector in the C41 host. (b) Di­thio­nite reduced the CO-bound form of CYP105D18.

Volume 8| Part 4| July 2021| Pages 684-694
ISSN: 2052-2525