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Figure 2
HPLC chromatogram and LC-MS spectra (inside the box) for (a) papaverine and (b) tetra­hydro­palmatine. The reaction (above) was performed with 400 µM substrate and 3 µM CYP105D18 in the presence of 40 mM H2O2 for 1 h. A similar reaction condition was used for the control (below), with the use of 3 µM heat-deactivated CYP105D18 (positive control) and without the CYP105D18 (negative control). The positive and negative controls showed a similar HPLC chromatogram without any product formation.

Volume 8| Part 4| July 2021| Pages 684-694
ISSN: 2052-2525