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Figure 3
Heme oxidation pattern of CYP105D18 in the presence of different concentrations of H2O2. (a) 1 mM, (b) 5 mM, (c) 10 mM, (d) 20 mM, (e) 40 mM, (f) 100 mM and (g) 200 mM H2O2. The concentration of CYP was fixed at 3 µM. The decrease in the Soret peak (417 nm) was measured at 90 s intervals for 30 min. Optimization of H2O2 concentrations for in vitro papaverine N-oxidation by CYP105D18 (h). The reaction conditions were as follows: 3 µM CYP, 400 µM substrate and 5–800 mM H2O2. The means and standard deviations were obtained from three independent reactions with similar conditions.

Volume 8| Part 4| July 2021| Pages 684-694
ISSN: 2052-2525