Figure 4
(a) Time-dependent conversion of papaverine to papaverine N-oxide. Conversion was measured every 5 min for 1 h using 1 µM CYP. The reactions were initiated by 40 mM H2O2. (b) Hyperbolic fit for papaverine N-oxide. (c) Hyperbolic fit for tetrahydropalmatine (THP). Average Km and kcat values were calculated with standard deviations from three independent experiments performed under similar conditions. |