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Figure 4
(a) PVM images of a riboA crystal (8 × 6 µm) in the presence of 10 mM adenine ligand. A crystal defect, which serves as the point of origin for the phase transition, is indicated by the green arrow. (b) Averaged intensity of crystal birefringence for each spatially averaged (10 × 10 pixels) superpixel in the selected ROI (80 × 80 pixels, green square) plotted versus time. (c) The first derivative of the intensity plot in (b). (d) Histogram of the time values of the first-derivative peaks for all superpixels (64), revealing T1, T2 and T3. As the addition of ligand causes disturbance to birefringence intensity and view of the crystal under the microscope, measurements prior to 10 s were omitted from the analysis.

IUCrJ
Volume 8| Part 4| July 2021| Pages 655-664
ISSN: 2052-2525
https://doi.org/10.1107/S2052252521004826