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Figure 8
(a)–(c) Schematic representation of the mechanism of maturation of Class 2 asparaginases. The process is started by the nucleophilic attack of (101)Thr on the scissile bond (100)Gly-(101)Thr (black). The reaction needs a water molecule (red) and assistance of the general base B. The oxyanion hole (orange) is made by (Na)Asn from the stabilization loop and another water molecule (orange). At the end of the maturation process, the protomer is cleaved into two subunits: α (green) and β (blue).

Volume 8| Part 4| July 2021| Pages 514-531
ISSN: 2052-2525