view article

Figure 2
Application of the reconstruction method to helical structures. (a), (e) The starting model structures of double-stranded DNA (phosphate positions only) and the 28/13 helix of F-actin, respectively. The gray continuous helices and the central axes are shown for assisting purposes. (b), (f) Expected fiber diffraction patterns, consisting of helix-derived layer-line reflections. (c), (g) CAPs of the model structures. (d), (h) The 3D structures reconstructed from the CAPs alone. (i) A goodness of fit of the original and reconstructed F-actin structures, which depends on the magnitude of permissible errors set in the computer program. The scale bar in (a) also applies to (c) and (d), and the bar in (e) also applies to (g), (h) and (i). The values on the scale bars in (b) and (c) are d spacings (nm). Figs. S2 and S3 of the supporting information show how internal vectors in the 3D structures [(a) and (e)] are picked up in their CAPS [(c) and (g)].

Volume 8| Part 4| July 2021| Pages 544-548
ISSN: 2052-2525